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Cryptogein-induced cell cycle arrest at G2 phase is associated with inhibition of cyclin-dependent kinases, suppression of expression of cell cycle-related genes and protein degradation in synchronized tobacco BY-2 cells.

Identifieur interne : 001768 ( Main/Exploration ); précédent : 001767; suivant : 001769

Cryptogein-induced cell cycle arrest at G2 phase is associated with inhibition of cyclin-dependent kinases, suppression of expression of cell cycle-related genes and protein degradation in synchronized tobacco BY-2 cells.

Auteurs : Ryoko Ohno [Japon] ; Yasuhiro Kadota ; Shinsuke Fujii ; Masami Sekine ; Masaaki Umeda ; Kazuyuki Kuchitsu

Source :

RBID : pubmed:21565910

Descripteurs français

English descriptors

Abstract

Induction of defense responses by pathogens or elicitors is often accompanied by growth inhibition in planta, but its molecular mechanisms are poorly understood. In this report, we characterized the molecular events that occur during cryptogein-induced cell cycle arrest at G(2) phase in synchronously cultured tobacco Bright Yellow-2 (BY-2) cells. Concomitant with the proteinaceous elicitor-induced G(2) arrest, we observed inhibition of the histone H1 kinase activity of cyclin-dependent kinases (CDKs), which correlated with a decrease in mRNA and protein levels of CDKB1. In contrast, the amount of CDKA was almost unaffected by cryptogein even at M phase. Cryptogein rapidly inhibited the expression not only of positive, e.g. A- and B-type cyclins and NtCAK, but also of negative cell cycle regulators such as WEE1, suggesting that cryptogein affects multiple targets to inactivate CDKA to induce G(2) arrest by mechanisms distinct from known checkpoint regulation. Moreover, we show that CDKB1 and cyclin proteins are also rapidly degraded by cryptogein and that the proteasome-dependent protein degradation has a crucial role in the control of cryptogein-induced hypersensitive cell death.

DOI: 10.1093/pcp/pcr042
PubMed: 21565910


Affiliations:


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Le document en format XML

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<term>Cell Nucleus (drug effects)</term>
<term>Cell Nucleus (metabolism)</term>
<term>Cyclin-Dependent Kinases (antagonists & inhibitors)</term>
<term>Cyclin-Dependent Kinases (metabolism)</term>
<term>Cyclins (genetics)</term>
<term>Cyclins (metabolism)</term>
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<term>G2 Phase (drug effects)</term>
<term>G2 Phase (genetics)</term>
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<term>Molecular Sequence Data (MeSH)</term>
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<term>Plant Proteins (metabolism)</term>
<term>Proteasome Inhibitors (MeSH)</term>
<term>Protein Processing, Post-Translational (drug effects)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>S Phase (drug effects)</term>
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<term>Cyclines (métabolisme)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Facteurs de transcription (génétique)</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Gènes cdc (MeSH)</term>
<term>Inhibiteurs du protéasome (MeSH)</term>
<term>Kinases cyclines-dépendantes (antagonistes et inhibiteurs)</term>
<term>Kinases cyclines-dépendantes (métabolisme)</term>
<term>Maturation post-traductionnelle des protéines (effets des médicaments et des substances chimiques)</term>
<term>Mitose (effets des médicaments et des substances chimiques)</term>
<term>Mort cellulaire (MeSH)</term>
<term>Noyau de la cellule (effets des médicaments et des substances chimiques)</term>
<term>Noyau de la cellule (métabolisme)</term>
<term>Phase G2 (effets des médicaments et des substances chimiques)</term>
<term>Phase G2 (génétique)</term>
<term>Phase S (effets des médicaments et des substances chimiques)</term>
<term>Protéines d'algue (pharmacologie)</term>
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<term>Protéines végétales (métabolisme)</term>
<term>Protéines à fluorescence verte (MeSH)</term>
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<term>Régulation négative (MeSH)</term>
<term>Tabac (cytologie)</term>
<term>Tabac (effets des médicaments et des substances chimiques)</term>
<term>Tabac (enzymologie)</term>
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<term>RNA, Messenger</term>
<term>Transcription Factors</term>
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<div type="abstract" xml:lang="en">Induction of defense responses by pathogens or elicitors is often accompanied by growth inhibition in planta, but its molecular mechanisms are poorly understood. In this report, we characterized the molecular events that occur during cryptogein-induced cell cycle arrest at G(2) phase in synchronously cultured tobacco Bright Yellow-2 (BY-2) cells. Concomitant with the proteinaceous elicitor-induced G(2) arrest, we observed inhibition of the histone H1 kinase activity of cyclin-dependent kinases (CDKs), which correlated with a decrease in mRNA and protein levels of CDKB1. In contrast, the amount of CDKA was almost unaffected by cryptogein even at M phase. Cryptogein rapidly inhibited the expression not only of positive, e.g. A- and B-type cyclins and NtCAK, but also of negative cell cycle regulators such as WEE1, suggesting that cryptogein affects multiple targets to inactivate CDKA to induce G(2) arrest by mechanisms distinct from known checkpoint regulation. Moreover, we show that CDKB1 and cyclin proteins are also rapidly degraded by cryptogein and that the proteasome-dependent protein degradation has a crucial role in the control of cryptogein-induced hypersensitive cell death.</div>
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<affiliations>
<list>
<country>
<li>Japon</li>
</country>
</list>
<tree>
<noCountry>
<name sortKey="Fujii, Shinsuke" sort="Fujii, Shinsuke" uniqKey="Fujii S" first="Shinsuke" last="Fujii">Shinsuke Fujii</name>
<name sortKey="Kadota, Yasuhiro" sort="Kadota, Yasuhiro" uniqKey="Kadota Y" first="Yasuhiro" last="Kadota">Yasuhiro Kadota</name>
<name sortKey="Kuchitsu, Kazuyuki" sort="Kuchitsu, Kazuyuki" uniqKey="Kuchitsu K" first="Kazuyuki" last="Kuchitsu">Kazuyuki Kuchitsu</name>
<name sortKey="Sekine, Masami" sort="Sekine, Masami" uniqKey="Sekine M" first="Masami" last="Sekine">Masami Sekine</name>
<name sortKey="Umeda, Masaaki" sort="Umeda, Masaaki" uniqKey="Umeda M" first="Masaaki" last="Umeda">Masaaki Umeda</name>
</noCountry>
<country name="Japon">
<noRegion>
<name sortKey="Ohno, Ryoko" sort="Ohno, Ryoko" uniqKey="Ohno R" first="Ryoko" last="Ohno">Ryoko Ohno</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

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